Column:

GP-C8, 5 µm, 120 Å, 4.6 × 250 mm

Mobile phase:

(3 mM β- cyclodextrin + 10 mM KH₂PO₄) : ACN = 60 : 40 (v/v)

Flow rate:

1.0 mL/min

Temperature:

45 ^oC

Detection:

UV 205 nm

Injection Volume:

20 µL

Sample:

Tromethamine salt （氨丁三醇盐）

MW: 580~620; about pH 3; unstable below pH 1.2.